Figure 4
From: Potent inhibition of rhabdoid tumor cells by combination of flavopiridol and 4OH-tamoxifen
Effect of caspases on flavopiridol- and 4OH-Tam-induced cell cycle arrest and apoptosis: A. Diploid cell cycle profile determined by FACS analysis of MON cells treated with Flavopiridol and 4OH-Tam for two days in the presence of pan-caspase inhibitor Z-VAD-FMC (Compare to Figure 2A); B. Percentage of MON cells at sub-G1 when exposed for two days to flavopiridol and 4OH-Tam, in the absence and presence of pan-caspase inhibitor Z-VAD-FMC (CI). C. Kinetics of induction of caspase 3/7 activity in MON cells by flavopiridol and 4OH-Tam. The diagram illustrates the fold increase in caspase 3/7 activity at various time points after treatment. F100 = 100 nM flavopiridol; T5 = 5 μM 4OH-Tam.