Coactivator overexpression increases proliferation and resistance to PPAR ligands in human breast cancer cell lines. (A) Triplicate cultures of stable CBP and SRC-1 expressing clones were grown in vitro for 6 days and compared to proliferation rates of neomycin resistant control cells (neo). Error bars indicate SEM of 3 independent experiments. (B, C) SRC-1, CBP, and neomycin resistant (neo) stable MCF7 or MDA-MB-468 clones were treated with 100 μM clofibrate (clo) or ciglitazone (cig) alone or in combination with 100 nM AGN194204 and grown in vitro for 6 days. Cells were counted with a hemacytometer. Error bars indicate SEM of 3 independent experiments.