COX-2 expression and phosphorylation of Akt at Ser473, BAD at Ser136 and caspase-9 at Ser196 in human breast tumors. IHC analysis of two invasive carcinomas for COX-2 (A,E), Ser473-phosphorylated Akt (B,F), Ser136-phosphorylated BAD (C,G), and Ser196-phosphorylated caspase-9 (D,H). Tumor to the left was negative for all markers (A,B,C,D). Tumor to the right shows that COX-2 is expressed in the cytoplasm of tumor cells with some cells showing marked perinuclear localization of COX-2 protein (E). Immunostain intensity is moderate to strong. Same tumors shows increased phosphoryation of Akt in the cytoplasm and along the inner cell membrane (F), and moderate to strongly increased phosphorylation of BAD (G) and caspase-9 (H), with a distinct immunostain suggesting a partly mitochondrial localization of the phosphorylated proteins (insets). Magnification: 200×. Counterstain: Methyl Green.