Table 3 Cyclin D2 and PTCH1 expression and promoter hypermethylation analysis in astrocytic cell lines
Astrocytic Cell lines | Cyclin D2 expression | Cyclin D2 Expression after 5'aza+TSA | Cyclin D2 promoter hypermethylation analysis by MCA-MSP | Cyclin D2 promoter hypermethylation Analysis by MCA-Meth | Cyclin D2 Hypermethylation by both techniques | No Hypermethylation in Cyclin D2 promoter | PTCH1 expression in glioma cell lines | PTCH1 promoter hypermethylation by MCA-MSP |
|---|---|---|---|---|---|---|---|---|
U87MG | + | + | √ | √ | √ |  | +/- |  |
A172 | - | + |  |  |  | √ | - |  |
LN405 | + | + |  |  |  | √ | + |  |
SW1783 | - | + | √ |  |  |  | - |  |
T98G | - | ++ | √ | √ | √ |  | ++ |  |
SW1088 | + | + |  |  |  | √ | ++ |  |
CCF-STTG-1 | - | + | √ |  |  |  | ++ | √ |
GOS-3 | - | ++ | √ | √ | √ |  | ++ |  |