Reversal of Cyclin D2 expression after treatment with demethylating agent 5-Aza-2'-deoxycytidine and TSA. Figure 6A: All 5 cell lines (A172, SW1783, T98G, CCF and GOS-3) show reversal in the expression of Cyclin D2 by RT-PCR. -: before treatment; +: after treatment. Figure 6B: Reversal of expression of Cyclin D2 transcript in the 5 cell lines after the treatment with 5-Aza-2'-dC and TSA, by qRT-PCR. Figure 6C: Comparative fold expression of Cyclin D2 after the treatment with 5-Aza-2'-dC and TSA. Some astrocytic cell lines even showed 200 fold increase in Cyclin D2 expression (p = 0.0014).