|
PRIMER Name
|
Nucleotide sequence
|
Region
|
Identified SNP
|
Number of cycles
|
Melting Temperature
|
Enzyme#
|
|---|
|
-1290AG
|
F
|
5'
|
TGCTGTCATTTTCCTGTAATGC
|
3'
|
PR
|
rs689465
|
34
|
60°C
|
PciI
|
|
-1195AG
|
R
|
5'
|
TTTCTCTCCCTGATGCGTGG
|
3'
| |
rs689466
| | |
AluI
|
|
AM1*
|
F
|
5'
|
GCTGTCAAAATCTCCCTTCC
|
3'
|
PR
|
rs20415
|
30
|
58°C
| |
| |
R
|
5'
|
CCACGCATCAGGGAGAGAAA
|
3'
| | | | | |
|
AM2*
|
F
|
5'
|
AACCAAAATAATCCACGC
|
3'
|
PR
| |
30
|
63°C
| |
| |
R
|
5'
|
CAAGGAGGGGGTGAAG
|
3'
| | | | | |
|
-765GC
|
F
|
5'
|
CTTTGTCCATCAGAAGGCAGG
|
3'
|
PR
|
rs20417
|
35
|
63°C
|
AciI
|
| |
R
|
5'
|
TAGAGGGTCGAGGAAGTCACG
|
3'
| | | | | |
|
AM3*
|
F
|
5'
|
TTACCTTTCCCGCCTCTC
|
3'
|
PR
|
rs20419
|
30
|
58°C
| |
| |
R
|
5'
|
GCGTCGTCACTAAAACATAAAAC
|
3'
| | | | | |
|
AM4*
|
F
|
5'
|
GCTATGTATGTATGTGCTGC
|
3'
|
PR
| |
31
|
55°C
| |
| |
R
|
5'
|
GAACTGGCTCTCGGAAGC
|
3'
| | | | | |
|
AM5*
|
F
|
5'
|
CGGTATCCCATCCAAGGC
|
3'
|
PR
|
rs5270
|
31
|
55°C
| |
| |
R
|
5'
|
CAGTGAGCGTCAGGAGCA
|
3'
| |
rs20424
| | | |
|
8473TC
|
F
|
5'
|
CTGTTGCGGAGAAAGGAGTC
|
3'
|
3'-UTR
|
rs5275
|
30
|
58°C
| |
| |
R
|
5'
|
TCAAACAAGCTTTTACAGGTGA
|
3'
| | | | | |
|
9850AG**
|
F
|
5'
|
CGTTCCCATTCTAATTAATGCCCTT
|
3'
|
3'-UTR
|
rs4648298
|
34
|
50°C
|
AluI
|
| |
R
|
5'
|
TGTGTCAAGCACTGTGGGTTTTAAT
|
3'
| | | | | |
|
10335GA
|
F
|
5'
|
TTTGGGAAGAGGGAGAAAATGA
|
3'
|
3'-UTR
|
rs689469
|
30
|
56°C
| |
| |
R
|
5'
|
TATGCGAATGTTTCAGTGCC
|
3'
| | | | | |
- * 15; ** 16; PR: Promoter Region; 3'-UTR: 3';Untranslated Region
-
# Enzymes used for PCR-RFLP. All other analyses were performed by dHPLC.
