Figure 3

Effects of HIF-1α-siRNA and CTM in U251MG and U343MG Cells: Western Blot Analyses HIF-1α, CAIX and PARP protein Levels. HIF-1α/CAIX protein expression and PARP cleavage following treatment with 75 nM HIF-1α-siRNA/CTM or 75 nM control siRNA/DMSO in comparison with untreated cells. Specificity of siRNA on HIF-1α knockdown and effects of CTM on HIF-1 activity under normoxic and hypoxic conditions. A (U251MG), Treatment with HIF-1α-siRNA caused induction of apoptosis under hypoxic conditions. CAIX expression is inhibited by HIF-1α silencing under normoxia and hypoxia. B (U343MG), Transfection with HIF-1α-siRNA caused induction of apoptosis under normoxic and hypoxic conditions. CAIX expression is attenuated by HIF-1α silencing under normoxia and hypoxia. C (U251MG), CTM enhanced the normoxic and hypoxic HIF-1α protein expression. Treatment with CTM induced apoptosis under normoxic and hypoxic conditions. CTM attenuated the normoxic and hypoxic CAIX expression. D (U343MG), Treatment with CTM decreased the normoxic and hypoxic HIF-1α protein expression. Exposure to CTM caused induction of apoptosis under hypoxic conditions. CTM attenuated the normoxic and hypoxic expression of CAIX. β-Actin served as a loading control.