Figure 5
From: Activated CD4+T cells enhance radiation effect through the cooperation of interferon-γ and TNF-α
Enhancement of radiosensitizing effect of IFN-γ by TNF-α. On day -2, Hela cells were pre-sensitized for 2 days with IFN-γ, TNF-α, IFN-γ+TNF-α, combination of 10 cytokines (Cyt), or aCD4S, followed (or not) on day 1 with 4 Gy γ-irradiation. Cells were then plated in 96-well plate at 5 × 103 cells/well in 200 μl CM and cell growth inhibition was determined on day 5 by WST-1 assay. All experiments were performed in triplicate and repeated at least 2 times. Data represent mean values of each triplicate ± S.D.