14-3-3σ expression in paired normal and cancer pancreas. A, Western blot analysis. Lysates from fresh frozen matching human normal (N) and pancreatic ductal adenocarcinoma (C) tissues were separated by SDS-PAGE followed by Western blot analysis of 14-3-3σ and GAPDH control. The relative level (R.L.) of 14-3-3σ in each sample was measured and normalized to that of GAPDH. The level of 14-3-3σ in each normal tissue was set to 1. B, summary of Western blot analysis. The relative levels of 14-3-3σ in normal and cancer tissues were graphed with the median level in each group marked by (-). Statistical analysis was done using Student T-test.