NF-κB activity is upregulated with Ad5hTRAIL infection but down-regulated with AdIKKβKA in A549 cells. A549 cells were simultaneously infected with AdNFkBLuc, Ad5hTRAIL, and increasing doses of AdIKKβKA construct for 30 h. As a negative control, AdCMVLacZ infection was utilized. The different constructs used in the infection and the MOI values represented in DNA particles/cell are listed on the X-axis. Luciferase activity expressed as Relative Light Units per microgram protein is depicted on the Y-axis, where the values represent the mean (± SEM) of six independent data points (n = 6). * p < 0.05.