Figure 3

Characteristics of DC subsets in RCC. By double immunfluorescence investigations the immature CD1a+ DCs (indocarbocyanine 3 (Cy3) - red fluorescence) in the tumour bed show co-expression for Langerin (indocarbocyanine 2 (Cy2) - green fluorescence; A, 400x). CD83+ DCs (Cy3 - red fluorescence) of the tumour border show co-expression for the maturation-associated antigens DC-LAMP (B), CD40 (C), and nuclear-expressed RelB (D, all 400x, all Cy2 - green fluorescence). CD83+ DCs (Cy3 - red fluorescence) form clusters with CD3+ T-cells (E, 400x, Cy2 - green fluorescence). T cells adjacent to the CD83+ mature DCs (Cy3 - red fluorescence) show an increased proliferation as indicated by nuclear Mib-1 expression (F, 400x, Cy2 - green fluorescence).