Figure 1

Expression status of NORE1A and NORE1B in human colorectal tissues and cancer cell lines. Expression of NORE1A and NORE1B mRNA in normal colorectal epithelial tissues (A). Semi-quantitative RT-PCR was performed using exon-specific primers and 10 μl of the PCR products were resolved on a 2% agarose gel. GAPDH was used as an endogenous control. Frequent reduction of NORE1A and NORE1B expression in colorectal cancer cell lines (B). An immunoblting assay was performed using NORE1A-specific antibody and chemiluminescence detection. Expression of NORE1A and NORE1B in primary colorectal carcinoma tissues (C). Tumor-specific reduction of NORE1A and NORE1B expression (D). NORE1 expression in tumor (T) and adjacent noncancerous (N) tissues were compared using matched tissue sets obtained from the same patients (P).