Figure 3

Downregulation of the i10ce variant by NMD. (A) Upf1 depletion increases levels of the chTERT splice variant i10ce. Levels of i10ce are expressed relative to total chTERT levels. Peak areas of i10ec were obtained by capillary electrophoresis analysis of the PCR products targeting exon 10, generated from cDNA from 1 well of P6 transfected with 25 pmol or 50 pmol of siUPF-1. The histogram shows the mean and standard deviation obtained from 4 biological analyses. All values are expressed as a fold difference with respect to "no silencing" (NS), for which the value was fixed at 1. (B) siUpf1 knockdown of Upf1. The histogram shows Upf1 mRNA levels, calculated by RT-PCR analysis. Upf1 levels in each sample were normalized with respect to GAPDH levels for the corresponding sample. The values obtained for "no silencing" (NS) LMH cells were set at 100%.