Figure 1

RT-PCRs determination of selected genes in BC tissues. Samples from tumor (T) and nontumor (NT) tissues were treated and analyzed as described in Methods. The PCR products were fractionated by 1.2% agarose gel electrophoresis. (A) Representative RT-PCR experiments from three independent replicas are shown. (B) Densitometric analyses of PCR gel bands, obtained from patients, represent the measurements done on three separate experiments. Data were pooled and analyzed to produce an average level of expression. Alternatively, gene of interest/β-actin or/GADPH ratios (normalized for each experimental time point) have been used for normalization. An arbitrary densitometric unit bar graph (SD) is shown. The p values (< 0.01) were measured with the Student's t test.