Expression of purified rhFNHN29 and rhFNHC36 polypeptides. (A), Schematic structure of FN. FN is made up of a series of repeating homology units of three different types (FNI, FNII and FNIII). Yellow rectangles represent FNI modules, blue rhombus represent FNII modules and red ovals represent specific FNIII modules. RhFNHN29 containing N-terminal heparin-binding domain (HBD) is comprised of the FNI 1-5 modules, and rhFNHC36 containing C-terminal HBD is comprised of the FNIII12-14 modules. (B), Purified rhFNHN29 and rhFNHC36 polypeptides were subjected to SDS-PAGE and visualized by Coomassie Blue staining. (C), The purified rhFNHN29 and rhFNHC36 polypeptides were analysed by Western blot. Immunoreactive fragments of 27.9 kDa and 31.0 kDa indicated their antigenicity.