Figure 3

Nuclear condensation was detected by tamoxifen treatment both in MCF-7 and T47 D cells. (A) MCF-7 and T47 D cells were seeded in a 4-chamber slide glass and incubated overnight. The cells were treated with or without 40 μM of tamoxifen for 20 h, fixed, and stained with Hoechst 33258. The arrows show nuclear condensation. (B) Percentage of cells intensely fragmented and condensed in nuclei with Hoechst 33258 were counted as positive cells. About 100 total cells were counted in individual ten random microscopic fields at ×40 magnification. Each value represents the mean + SE (bars) of two independent experiments *p < 0.05, according to the t-test.