Figure 1

The protein levels of claudin-1 were increased in MCF-7 cells treated with tamoxifen. (A) MCF-7 and T47 D cells were treated with the control treatment (buffer alone: Mock) for 20 h or 20, 30, or 40 μM of tamoxifen for 20 h, cell lysates were prepared and subjected to Western blot analyses for cleaved PARP, claudin-1, claudin-4, claudin-7, E-cadherin, cleaved caspase-8, and actin. One representative of at least three independent experiments with similar results is shown. (B) The intensity of the bands for cleaved PARP, claudin-1 and E-cadherin in untreated and tamoxifen (40 μM) - treated cells were quantified. (C) MCF-7 cells were treated with or without 1, 10 or 20 μM of tamoxifen for 48 h, cell lysates were prepared and subjected to Western blot analyses for cleaved PARP, claudin-1 and actin. One representative of at least three independent experiments with similar results is shown. (D) The intensity of the bands for cleaved PARP and claudin-1 in untreated and tamoxifen (40 μM) - treated cells were quantified.