Figure 2

17q12-q21-amplicon as the most significant on 17q. Unsupervised hierarchical clustering of genome copy number profiles measured for 54 ERBB2-aplified primary breast tumors by aCGH on 5,574 17q probes. Legend is similar to Figure 1. The bar to the left indicates chromosome 17q location with centromere to the top and qter to the bottom. Below the dendrogram, name of tumors are given and the rows indicate their ER and PR IHC status (black square, ER+ or PR+; white square, ER- or PR-). Color codes and corresponding legends are indicated in the box located to the top right. On the right, combining the CNA frequency and gene amplification level, the GISTIC algorithm plotted the score index as a function of chromosome location. AATF and STAT3 delineate the genomic fragment that defined the ERBB2-amplicon. The green line indicates the threshold of significance for the score. Previous studies have reported that the 17q arm is the site of multiple amplicons. They are indicated by black bars at the right of the plot score. The Figure shows only 17q12-q21 (pink box) centered on the ERBB2 locus as the significant 17q amplicon (p < 0.001).