RAD001 induces cell cycle arrest and apoptosis in BGC823 cells. (A) BGC823 cells were exposed to various concentrations of RAD001 for 48 h. (B) RNA were extracted from cells. Cyclin D1 and C-myc expression level were detected by RT-PCR, actin as a loading control. (C) cells were incubated for 48 hrs with indicated doses of RAD001 before staining with annexin V-FITC. The percentages of apoptotic cells are displayed. (D) Cells were incubated for 48 hrs with indicated doses of RAD001. Cells were collected, lysed and subjected to Western blot analysis with Bcl-2 and p53 antibodies. GAPDH was used as a loading control.