Figure 3

Detection and analysis of the MLL-CT45A2 fusion transcript. (A) RT-PCR analysis with a MLL sense primer located in MLL exon 8 and one CT45A2 sense primer located in CT45A2 exon 2 (lane 1). Lane 2 - 100 bp molecular marker. (B) Partial sequence of the junction of the MLL-CT45A2 e9e2 chimeric mRNA, showing the nucleotide sequence of the fusion transcript containing 6 bp (in red) from the 5' UTR of CT45A2 exon 2 coding for two additional amino acids. The arrow shows the in-frame fusion between MLL exon 9 and CT45A2 exon 2. (C) Partial sequence of the junction of the MLL-CT45A2 e8e2 chimeric mRNA, showing the nucleotide sequence of the fusion transcript. The arrow shows the out-of-frame fusion between MLL exon 8 and CT45A2 exon 2 that leads to a premature stop codon 14 nucleotides downstream of fusion breakpoint.