Effects of topical treatments including steroid and barrier film spray on the radiation- induced injury by immunochemical analysis. Immunohistochemical analyses using an antibody against TGF-β (Magnification ×400) were performed on murine cutansous tissues 20 days after irradiation. Three mice from each group were examined. Representative slides are shown for (a) unirradiated control mice, (b) irradiated mice at 20 days after 15 Gy irradiation, (c) steroid cream-treated mice at 20 days after 15 Gy irradiation, (d) Barrier film spray-treated mice at 20 days after 15 Gy irradiation. Increased TGF-β immunoreactivity was detected after irradiation. Topical treatment attenuated the inflammatory response, especially in steroid cream treated mice. Duplicate experiments were performed for the analysis.