Figure 1

Hyaluronidase extractability. A. Up. Typical hyaluronan zymography pattern of the three sequential extracts of the samples, macroscopically normal (N) and cancerous (C) of patients with colorectal cancer. The extracts are PBS (1), 4 M GdnHCl (2) and 4 M GdnHCl - 1% Triton X-100 (3). Down. Semi-quantitative representation of the zymography experiments. B. Up. Hyaluronidase activity in PBS extracts from healthy subjects (H), macroscopically normal (N) and cancerous (C) specimens of patients. Down. Semi-quantitative representation of the zymography experiments. The arrow indicates the migration of the main lysis band.