Immunoblot validation of differentially expressed genes. A. Relative gene expression for 16 selected genes by microarray or real time RT-PCR analysis. B. Protein expression for these gene products by immunoblotting. Representative blots from 3-5 independent experiments are shown, including a beta-actin loading control. Myc protein expression was only examined at the 4 hr timepoint and was not detected at other time-points. Relative quantification of immunoblotting data is shown in Additional file 6.