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Table 1 Sources and dilutions of primary antibodies related to the Vitamin D metabolism used in this study for immunohistochemistry

From: Alterations in Vitamin D signalling and metabolic pathways in breast cancer progression: a study of VDR, CYP27B1 and CYP24A1 expression in benign and malignant breast lesions Vitamin D pathways unbalanced in breast lesions

Antibody Clone Manufacturer Time of incubation (min) Dilution Antigen retrieval (min)
VDR 9A7γE10.4 Calbiochem, Germany overnight 1:50 30
CYP27B1 C12 Santa Cruz, USA 60 1:200 30
CYP24A1 C18 Santa Cruz, USA overnight 1:75 30