Table 1 Sources and dilutions of primary antibodies related to the Vitamin D metabolism used in this study for immunohistochemistry
Antibody | Clone | Manufacturer | Time of incubation (min) | Dilution | Antigen retrieval (min) |
|---|---|---|---|---|---|
VDR | 9A7γE10.4 | Calbiochem, Germany | overnight | 1:50 | 30 |
CYP27B1 | C12 | Santa Cruz, USA | 60 | 1:200 | 30 |
CYP24A1 | C18 | Santa Cruz, USA | overnight | 1:75 | 30 |