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Figure 4 | BMC Cancer

Figure 4

From: Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF) as a reference

Figure 4

Standard curves for MS-qPCR with conventional primers. MS-qPCR standard curves with serial dilution mixtures of SssI-treated DNA:untreated DNA (100%, 50%, 10%, 1%, 0.1%, 0.01%, 0.001%; each containing 1.5 μg of template DNA) for conventional primers[35] specific for mMGMT. Conventional primers for mMGMT show lower PCR efficiency (slope: -4.339; efficiency: 69%) than LNA modified primers (see Fig.3). The analytical detection limit with the use of conventional primers was 0.1%.

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BMC Cancer

ISSN: 1471-2407

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