Transplantation microenvironment influences DNA methylation in SRC tumors. Pyrosequencing revealed that Satellite 1 DNA was hypomethylated compared to DNA from normal rat cartilage (control tissue). The satellite 1 DNA elements in the subcutaneous SRC tumor and the tibia SRC tumor were hypomethylated compared to the DNA in the lung SRC tumor. The graph illustrates the average Satellite 1 DNA methylation calculated from a pool of tissues from each transplantation site. For each transplantation site, tumor tissue was pooled from at least 10 separate animals. Bars represent the average DNA methylation % of technical replicates of the pooled tissue samples, and error bars represent the standard deviation of these replicates. P-values are indicated for comparisons between the specific sample and the "Normal rat cartilage" sample using Dunnett method in the analysis of variance (ANOVA). We attributed statistical significance to p values ≤ 0.05.