Minimal Regions of Overlap in Copy Number. A amplified region on chromosome 8q21.11 is shown in 4a. The minimal region of overlap has an average of 4.9 copies in 19 samples. This small region is 13 KB (8: 75237536-75250960) and is surrounded by an amplification or gain in 7-18 samples. This extended region is 90 KB and begins at 8p11 and extends to the telomere (8:41489634- 133808163). 4b shows an novel copy number region of gain at 5p15.33 where two minimal regions of overlap were detected. The first extends 101 KB (5:1836735-1938410) and the second is 19 KB (5:3180419-3199829) while the entire amplified region extends 5.6 MB from 5p15.33-p33.2 (5:165712-5779631). The bottom trace shows the SNP intensity plot for the region. The larger dots are smoothed data and the fine dots are unsmoothed. The first region has a mean of 3.5 copies and the second has 4.7.