Phenotyping breast cancer cell lines EM-G3, HCC1937, MCF7 and MDA-MB-231 using 2-D electrophoresis and affinity chromatography for glutathione-binding proteins

Table 4 Relative intensities of spots in the gels from GST-binding fractions

Spot no.	Accession no.	Protein name	Relative intensity of spots^a
			EM-G3	HCC1937	MDAMB231	MCF7
1	P09211	GST P1-1	49	6	48	6
2	P09211	GST P1-1	16	-	7	-
3	P09211	GST P1-1	10	-	11	3^b
4	P21266	GST M3-3	2	3	6	12
5	P24534	EF 1-β	11	26	5	2
6	P26641	EF 1-γ	8	8	3	1
7	P60709	β-actin	17	34	24	32
8	P16152	Carbonyl reductase [NADPH] 1	15	22	1	-
9	O43813	LanCL 1	4	9	14	-

For each protein spot we show the accession number in the Swiss-Prot database, the protein name or its abbreviation. Relative intensities of spots in the gels were estimated by PDQuest analysis. Protein names are abbreviated as GST = glutathione S-transferase, EF = elongation factor, LanCL 1 = LanC-like protein 1.
a) The values were normalized to total density in the gel image and are expressed as thousandths of total density.
b) The measured density corresponds to a proportional part of a diffuse spot that overlaps the respective position of spot no.3 (Figure 3).

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