Figure 2

Leptin promotes hTERT expression and TA in HepG2 cells. (a) Total RNA was isolated and its expression was evaluated using real-time RT-PCR, to determine changes in the level of hTERT mRNA expression after normalization to PBGD expression. All data were presented as a fold induction relative to untreated cells. Columns, mean of three independent experiments done in triplicate; bars, SD; *, p < 0.05 compared to untreated cells, (b) Telomerase activity after leptin and leptin siRNA treatment as mentioned above.