Methylation status of individual CpG sites within Alu elements in the human genome and Alu hypomethylation in gastric carcinomas

Table 2 Bioinformatic analysis of Content and distribution of Alu subfamilies among the PCR-1 and PCR-2 products Table legend text.

*Alu*subfamily	Bioinformatic analysis with the database^a			Analysis of bisulfite clone sequencings
	Average CpG no.^b	Copy no. of *Alu*	Copy no. of *Alu*matched to the primers for PCR-1	Clone no. of *Alu*obtained in the PCR-1 products	Total clone no. of *Alu*with methylated-CpG number ≥ 4 in the PCR-1 products	Clone no. of *Alu*obtained in the PCR-2 products^c
AluJ	16.3	29508 (6.2%)	28889 (6.1%)	52 (12.2%)	14 (6.3%)	3 (5.6%)
AluS	22.4	350888 (73.7%)	350419 (73.6%)	296 (69.3%)	156 (70.3%)	30 (55.6%)
AluY	24.5	95756 (20.1%)	91680 (19.3%)	79 (18.5%)	52 (23.4%)	21 (38.9%)
(Total)	22.4	476152 (100%)	470988 (100%)	427 (100%)	222 (100%)	54 (100%)

^a476152 copies of Alu elements extracted bioinformatically from the NCBI database of human genome (C) as described (Ref. [13]);
^b within the full sequence of Alu elements as illustrated in Figure 1A;
^camplified from two representative samples with the primer set-2 after bisulfite modification

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