Figure 3
From: Human Sulfatase 2 inhibits in vivo tumor growth of MDA-MB-231 human breast cancer xenografts
Recombinant human Sulfatase 2 inhibits growth factor-induced ERK activation. MDA-MB-231 cells were treated with 250 nM rhSulf2 for 24 h prior to FGF (A) or HB-EGF (B) stimulation. Cell lysates were collected before growth factor addition (0 min), or 15, 30, and 60 minutes after addition for analysis of phospho-ERK1/2 and total ERK. The two phosphorylated p44 and p42 species of ERK are detected using phospho-ERK1/2 antibodies.