Sulfatase expression in a human breast cancer model and analysis of recombinant human Sulfatase 2. A) Human breast cancer cell lines MDA-MB-231 (MB 231) and MDA-MB-435 S (MB 435) were tested for expression of Sulfatase1 (hSulf1), Sulfatase2 (hSulf2), or β-actin by RT-PCR. B) RT-PCR was used to detect hSulf1, hSulf2 or both sulfatase transcripts in MDA-MB-231 after stable transfection with human sulfatase-containing plasmids. C) Coomassie staining of SDS-PAGE gel shows a prominent band at 120 KDa corresponding to purified rhSulf2 loaded in both lanes. D) Purified hSulf2 protein was incubated at 250 nM in medium or mouse serum, and activity assayed over time.