Figure 3

Metastatic abilities of MCF-7 and MCF-7-14 cells in mouse xenograft model. (A) In vivo scanning of EGFP-expressing breast cancer cells in mice at 4 weeks after implantation into the mammary fat pad. In an MCF-7-14-EGFP-bearing mouse, metastasis to the pancreas (arrowhead) was imaged, corresponding to the photo of the abdomen. (B) EGFP-positive region in panel A was analyzed by HE and MGP staining, or unstained sections were analyzed for EGFP fluorescence. Metastasized MCF-7-14-EGFP cells (M) invaded pancreatic parenchyma (P). (C) Representative pancreas metastasis 12 weeks after implantation of MCF7-14-EGFP cells. Pancreas metastasis showed strong stromal (S) and angiogenic responses (brown) closely intermingled with metastasized tumor cells (M).