Figure 5
From: SLUG/SNAI2 and Tumor Necrosis Factor Generate Breast Cells With CD44+/CD24- Phenotype
SLUG overexpression leads to changes in CD44 and CD24 expression pattern. A) SLUG expression in CD44+/CD24- cells of primary breast cancer compared to normal breast epithelium. These results were generated from the study in Ref 13 with a GEO accession number GDS2617. B) RT-PCR (MCF-7 cells) or qRT-PCR (MCF-10A cells) analysis of overexpressed genes. C) Flow cytometric analysis of different cell types for CD44 and CD24. A subpopulation of MCF-7-SLUG cells acquired the CD44+/CD24+ phenotype, whereas a subpopulation of MCF-10A-SLUG cells acquired the CD44+/CD24- phenotype.