Figure 1
From: SLUG/SNAI2 and Tumor Necrosis Factor Generate Breast Cells With CD44+/CD24- Phenotype
Growth properties of the CD44+/CD24- and CD44-/CD24+ subpopulations of MCF-10A cells. A) Flow cytometric analysis of MCF-10A cells labeled with CD44-FITC and CD24-PE antibodies. Total MCF-10A cells can be divided into three distinct subpopulations: CD44+/CD24-, CD44-/CD24+, and CD44+/CD24+. B) Representative growth characteristics of CD44+/CD24- and CD44-/CD24+ cells in 2 D culture (top panel) and 3D-matrigel cultures. Hematoxylin & Eosin staining was performed to characterize cells grown in matrigel. C) Proliferation rate of CD44+/CD24-, CD44-/CD24+, and CD44+/CD24+ cells. Proliferation rate was measured using bromodeoxyuridine incorporation assay. Representative data from two experiments, both done in duplicate with eight wells per cell type, are shown.