Effects of Siah1 expression on invasion of SKBR3 cells. A: The invasion of SKBR3 cells was studied in a modified Boyden chamber assay. A significant decrease in invasion was detected in transfected SKBR3 cells relative to control group. Cell numbers were counted in 10 individual high-powered fields. Columns, mean; bars, SD; *, p < 0.05. B: Representative photomicrographs Magnification ×20.