Figure 9

GPR110 protein overexpression in paraffin sections of lung cancer tissue. Immunohistochemistry on human lung cancer tissue arrays with consecutive sections, including normal controls, with antibodies LS-A2021 and LS-A2019. (A) Three peptides spanning amino acids 162-199 of GPR110. (B) Binding of LS-A2021 antibody to lung samples was competed with the three peptides. Peptide 2 decreased staining in GPR110-positive tissue samples, whereas lack of peptide, and peptides 1 and 3 did not. (C) Examples of cores staining with LS-A2021 only (type 1), with both antibodies (type 2), and with LS-A2019 only (type 3); there was no staining with IgG controls (not shown).