Isoform 2 is secreted. Western blots of immunoprecipitates of cell culture media from GPR110 transfections; developed with antibody HA.11. (A) L, lysate; M, immunoprecipitate from media. HA466 and HA1393, two versions of HA-tagged isoform 1; Iso2-HA and Iso3-HA, HA-tagged isoform 2 and 3, respectively. Arrow points to the secreted form of isoform 2. (B) PNGaseF treatment of lysate and media of cells transfected with Iso2-HA. Arrow points to the deglycosylated isoform 2.