Table 2 Primers used for amplification/sequencing and biotinylated oligonucleotide probes
Forward primer 5'-3' | Reverse primer 5'-3' | Size of amplified fragment lengths (bp) | |
|---|---|---|---|
HIF1α 1.1 | CACCTCTGGAC TTGCCTTTCCTTC | CACCAGCATCCAG AAGTTTCCTCAC | 351 |
aHIF1α 1.2 | ATGTGCAAGAC ACTGCATTCTTAG | CACCAGCATCCAG AAGTTTCCTCAC | 220 |
HIF1α 1.3 | TGGTGGTTACTC AGCACTTTTAGA | CACCAGCATCCAG AAGTTTCCTCAC | - |
HIF1α 10-13 | GGCAATGTCTCC ATTACCCACCG | CGCTTTCTCTGAGC ATTCTGCAAG | 791 |
HIF1α12 | GGTGGCATTACG AGTAGGTTCTTGT | AGGTCCCTATATC CCAATGGATGA | 180 |
HIF1α 13-15 | ACTCAAAGTCGG ACAGCCTCACCA | ATCCATTGATTGC CCCAGCAGTC | 381 |
Probe HIF1α 1.2 | Antisense, Biotin-GC AAGATATGTG CAAGACAC-Biotin | Sense, Biotin-GTGT CTTGCACAT ATCTTGC-Biotin | - |