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Table 2 Primers used for amplification/sequencing and biotinylated oligonucleotide probes

From: HIF1α isoforms in benign and malignant prostate tissue and their correlation to neuroendocrine differentiation

  Forward primer 5'-3' Reverse primer 5'-3' Size of amplified fragment lengths (bp)
HIF1α 1.1 CACCTCTGGAC TTGCCTTTCCTTC CACCAGCATCCAG AAGTTTCCTCAC 351
aHIF1α 1.2 ATGTGCAAGAC ACTGCATTCTTAG CACCAGCATCCAG AAGTTTCCTCAC 220
HIF1α 1.3 TGGTGGTTACTC AGCACTTTTAGA CACCAGCATCCAG AAGTTTCCTCAC -
HIF1α 10-13 GGCAATGTCTCC ATTACCCACCG CGCTTTCTCTGAGC ATTCTGCAAG 791
HIF1α12 GGTGGCATTACG AGTAGGTTCTTGT AGGTCCCTATATC CCAATGGATGA 180
HIF1α 13-15 ACTCAAAGTCGG ACAGCCTCACCA ATCCATTGATTGC CCCAGCAGTC 381
Probe HIF1α 1.2 Antisense, Biotin-GC AAGATATGTG CAAGACAC-Biotin Sense, Biotin-GTGT CTTGCACAT ATCTTGC-Biotin -
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BMC Cancer

ISSN: 1471-2407

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