Figure 2

The inconsistency of three biomarkers. The three biomarkers were not completely overlap, some IHC positive or FISH positive patients were mutation negative. A-C: Case 3, A: IHC positive(3+), B: FISH positive(amplification),C: mutation negative(one ascending curve indicated EGFR wild type). D-F: Case 4, D: IHC positive(2+), E: FISH negative(disomy), F: Curves for exon 19 using the Scorpions ARMS method, if only one ascending curve showed the wild-type, more than two ascending curves showed the mutation. Two curves in this case indicated mutation(the left curve represented wild type, the right curve represented mutant, exon 19 deletion). G-I: Case 5, G: IHC negative, H: FISH positive(high polysomy), I: Curves for exon 21 using the Scorpions ARMS method. Two ascending curves in this case indicated mutation (the left curve represented wild type, the right curve represented mutant, exon 21 L858R mutation)