Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate

Table 2 Fold increase in cytokine secretion by coculture of monocytes and MDA-MB-231, as compared to the secretion by the two types of cells cultured separately

Cytokines	Fold change
Group 1: cytokines secreted by Mφ and not by MDA-MB-231: Ratio coculture medium/Mφ medium
MCP-1	1.45
Group 2: cytokines secreted by MDA-MB-231 and not by Mφ Ratio: coculture medium/MDA-MB-231 medium
GM-CSF	2.82
Groα	2.96
IL-6	2.68
Group 3: cytokines secreted by both Mφ and MDA-MB-231 Ratio coculture/(Mφ medium cultured separately + MDA-MB-231 medium cultured separately)
Gro	1.80
IL-8	2.35
Group 4: cytokines secreted only in the coculture medium
ENA-78
IL-10
MCP-3

In every case, the difference of spot intensity between duplicate determinations was <10%. The mean of the 4 spots (2 membranes × 2 spots per membrane) was used for determining the intensity of expression of each cytokine.

ISSN: 1471-2407