Figure 3

Secretion of TNFα into the culture medium of LPS and IFNγ-treated Mφ, MDA-MB-231 and cocultures. Cultures were incubated in the absence (A) or presence (B) of 5 μM TM, the corresponding medium being renewed daily. In all cases, cells were stimulated on day 5 with 1 μg/ml LPS and 5 ng/ml IFNγ for 24 h. Supernatants from these stimulated Mφ, MDA-MB-231 and cocultures were then tested for the presence of TNFα by ELISA. Mean ± SEM of 3 independent experiments. * p < 0.05 between Mφ and coculture without TM; p < 0.01 between Mφ without TM and Mφ with TM; p < 0.05 between coculture without and with TM. Mann-Whitney test. TNFα was not detected in the supernatants of non-activated cells, as also observed by cytokine array.