Figure 4

Phenotypic effects of hypoxia and low glucose. Effect of hypoxia (light grey bars) or low glucose (dark grey bars) on (a) cell number and (b) apoptosis in ES and OS cell lines and (c) mitotic index in OS cell lines. Bar graph data is normalised to the normoxic control (white bars) for each condition: *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus control. (d) Proliferation assessed in ES cell lines by Western blot for Ki-67. N, normoxia; H, hypoxia; G, low glucose. Numerical values represent mean fold change in Ki-67 as assessed by densitometry:*, p < 0.05; **, p < 0.01 versus normoxia.