Figure 3

HIF siRNA in ES and OS cell lines. (a) Western blots showing expression of HIF-1α (120 kDa), HIF-2α (120 kDa) and Glut-1 (50 kDa) in response to siRNA targeting HIF-1α (H1), HIF-2α (H2), HIF-1α and HIF-2α (H12) or control siRNA (scr) in ES (SK-N-MC) and OS (143B) cells. Effects of HIF siRNA on (b) secretion of VEGF and (c) activation of the HRE-luciferase reporter construct and in response to hypoxia or low glucose in ES and OS cell lines. Bar graph data is normalised to the siRNA mock control (-) for each condition and represents pooled data from the entire panel of ES or OS cell lines. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus scr siRNA control. (d) Secretion of TGFα from ES cell lines in response to hypoxia (light grey bars) or low glucose (dark grey bars). *, p < 0.05; **, p < 0.01; ***, p < 0.001.