SPC-A-1sci cells display distinct phenotype consistent with EMT. A, Representative phase-contrast images of SPC-A-1sci and SPC-A-1 cells are shown. Magnification, 200×. B, Immunofluorescence staining of α-tubulin, E-cadherin, ZO-1, Vimentin and Fibronectin in SPC-A-1sci cells and SPC-A-1cells. The green/red signal represents the staining of the indicated proteins, and the blue signal represents the nuclear DNA staining by DAPI. Magnification, 600×. C, Quantitative-PCR analysis of the mRNA levels of E-cadherin, Vimentin and Fibronectin in SPC-A-1sci and SPC-A-1 cells. Results are expressed as mean ± SD; **, P < 0.01; *, P < 0.05. D, Expression of E-cadherin and vimentin were examined by immunohistochemical staining of serial sections from s.c. primary tumours of SPC-A-1 cells, SPC-A-1sci cells and also pulmonary metastatic nodules of SPC-A-1sci cells. Magnification, 200×.