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Figure 4 | BMC Cancer

Figure 4

From: miR-183 inhibits TGF-β1-induced apoptosis by downregulation of PDCD4 expression in human hepatocellular carcinoma cells

Figure 4

miR-183 transfectants were resistant to apoptosis induced by TGF-β1. (A) DAPI assay. Huh7 cells were transfected with NC RNA, siPDCD4, or miR-183 respectively, after 24 hours, TGF-β1 (5 ng/mL) was added. Cells were further incubated for 48 hours, fixed, and stained with DAPI. *P < 0.05 compared with Huh7 cells transfected with NC RNA. (B) Annexin-V assays. Huh7 cells were transfected and cultured as described in (A), then collected by trypsinization followed by centrifugation and stained with FITC conjugated Annexin-V and PI. (C) miR-183 and PDCD4 expression vector were cotransfected into Huh7 cells, after 24 hours, TGF-β1 (5 ng/mL) was added. Cells were further incubated for 48 hours, fixed, and stained with DAPI. * P < 0.05 compared with Huh7 cells transfected with control pCMV vector.

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