miR-183 inhibits the mRNA and protein expression of PDCD4. (A) HepG2 cells transfected with miR-183 compared with negative control transfected cells for 48 hours, the mRNA expression levels of three potential target genes were detected by qRT-PCR. (B) PDCD4 protein was detected by western blot using anti-PDCD4 antibody and anti-β-actin antibody as a control. (C) Correlation between expression levels of miR-183 and PDCD4. U indicates up-regulation of miR-183 in HCC tissues, and NU indicates down-regulation and no significant change of miR-183 in HCC tissues. The mean value is shown as a horizontal line. Statistical analysis was performed with Student's t-test.