Figure 4

HB-19 affects the malignant phenotype of melanoma TIII cells. A. Reduced colony formation in soft agar. TIII cells were cultured for twelve passages in the absence (control, histogram C) or presence of 1, 5 and 10 μM of HB-19 before assay for colony formation in the absence of further HB-19 treatment (***p < 0.001). B. Reduced tumorigenicity in mice. Tumor size was measured fourteen days after transplanting control (open circles, n = 14) or HB-19 precultured (black circles, n = 15) TIII cells. The graph corresponds to the results of two separate experiments. The mice transplanted with HB-19 precultured cells displayed tumors smaller than those treated with control cells (ANOVA, Mann Whitney test, p = 0.0276). C. Reduced lung metastasis in mice. Mice injected in the tail vein with TIII cells were treated (closed circles, n = 8) or not (open circles, n = 10) with HB-19, and the number of black lung macro-metastases was determined (ANOVA, Mann Whitney test, p = 0.0014).