Figure 6
Quantitative determination of matrix calcification and alkaline phosphatase activity in MSC exposed to dasatinib or E804. A: Matrix calcium content of untreated MSC (Control), and cells cultured with 10-8 M dasatinib, DAG or a combination of both treatments, was assessed after 7, 14 and 21 days. B: ALP activity in MSC cultured in control medium supplemented or not with 10-8 M dasatinib and/or DAG was measured after 7, 14 and 21 days. ALP activity (U, μmol p-nitrophenol released per min) was normalized. C: ALP activity in MSC exposed to 10-7 M E804 and/or DAG for 7, 14 and 21 days. ALP activity (U, μmol p-nitrophenol released per min) was normalized normalized for 105 cells. A, B, C: Results are presented as mean ± SEM from 4 independent experiments performed in duplicate; * p < 0.05, ** p < 0.01 and *** p < 0.001 versus the corresponding control, and comparing DAG versus Dasatinib+DAG or versus E804+DAG.