Figure 1
Quantitative analysis of matrix calcification and alkaline phosphatase activity during osteogenic differentiation of MSC. A: Calcium content of the extracellular matrix of control cell cultures (open bars) and DAG-treated cell cultures (black bars) was determined after 7, 14 and 21 days. B: ALP activity in MSC cultured in control (open bars) or osteogenic (black bars) medium was measured after 7, 14 and 21 days. ALP activity (U, μmol p-nitrophenol released per min) was normalized for 105 cells. A, B: Results are presented as mean ± SEM from 5 independent experiments performed in duplicate; *** p < 0.001 versus the corresponding control.